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BioVendor Instruments polyclonal antibodies against human periostin
Polyclonal Antibodies Against Human Periostin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+periostin+antibody/us12472241-208-10-15?v=BioVendor+Instruments
Average 94 stars, based on 36 article reviews

polyclonal antibodies against human periostin - by Bioz Stars, 2026-08

94/100 stars

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Image Search Results

Comparison of the expression of POSTN (in cells ( A ) and in stroma ( B )) and the expression of pro-angiogenic markers (VEGF-A ( C ); CD31 ( D ); CD34 ( E ); CD105 ( F )) between non-malignant lung tissue and cancer cells. The significance of the differences was determined using the Mann–Whitney U test. The images in ( G – K ) show punches from tissue microarrays illustrating an example of the immunohistochemical (IHC) reaction for each individual protein, as described above. Error bars represent the standard deviation (SD). *** p < 0.001.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Comparison of the expression of POSTN (in cells ( A ) and in stroma ( B )) and the expression of pro-angiogenic markers (VEGF-A ( C ); CD31 ( D ); CD34 ( E ); CD105 ( F )) between non-malignant lung tissue and cancer cells. The significance of the differences was determined using the Mann–Whitney U test. The images in ( G – K ) show punches from tissue microarrays illustrating an example of the immunohistochemical (IHC) reaction for each individual protein, as described above. Error bars represent the standard deviation (SD). *** p < 0.001.

Article Snippet: The following specific primary antibodies were used in the IHC experiments: anti-POSTN rabbit polyclonal antibody (1:200 dilution, NBP1-82472, Novus Biologicals, Littleton, CO, USA), monoclonal mouse anti-VEGF-A antibody (1:50 dilution + linker, VG1 clone, M7273, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD31 antibody (RTU, JC70A clone, IR610, Agilent Technologies, Santa Clara, CA, USA), monoclonal mouse anti-CD34 antibody (RTU, QBEnd/10 clone, IR632, Agilent Technologies, Santa Clara, CA, USA), and rabbit polyclonal anti-CD105 antibody (1:1000 dilution, 10862-1-AP, Proteintech, Manchester, UK).

Techniques: Comparison, Expressing, MANN-WHITNEY, Immunohistochemical staining, Standard Deviation

Bar charts showing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) across all cancer types with regard to the T-stage ( A , D ) and N-stage ( B , E ) of the TNM classification as well as the tumor stage ( C – F ). The significance of the differences was determined using the Kruskal–Wallis ANOVA test and the differences between individual groups were evaluated using the appropriate post hoc test. Error bars represent the standard deviation (SD). * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Bar charts showing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) across all cancer types with regard to the T-stage ( A , D ) and N-stage ( B , E ) of the TNM classification as well as the tumor stage ( C – F ). The significance of the differences was determined using the Kruskal–Wallis ANOVA test and the differences between individual groups were evaluated using the appropriate post hoc test. Error bars represent the standard deviation (SD). * p < 0.05; ** p < 0.01; *** p < 0.001.

Techniques: Expressing, Standard Deviation

Bar charts showing the expression of POSTN in cells ( A – D ) and in stroma ( E – H ), across all cancer types ( A , E ), in adenocarcinoma ( B , F ), and in squamous-cell carcinoma ( C , G ), with regard to the tumor grade. The significance of the differences was determined using the Kruskal–Wallis ANOVA test ( p < 0.001 in bar plots ( A – C , E – G )), and differences between individual groups were evaluated with the appropriate post hoc test. The results obtained for large-cell carcinoma were statistically insignificant ( D , H ). The images in ( I – Q ) show representative punches demonstrating the IHC reaction for POSTN in adenocarcinoma ( I – K ), squamous-cell carcinoma ( L – N ), and large-cell carcinoma ( O – Q ) with respect to the tumor grade. Error bars represent the standard deviation (SD). *** p < 0.001.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Bar charts showing the expression of POSTN in cells ( A – D ) and in stroma ( E – H ), across all cancer types ( A , E ), in adenocarcinoma ( B , F ), and in squamous-cell carcinoma ( C , G ), with regard to the tumor grade. The significance of the differences was determined using the Kruskal–Wallis ANOVA test ( p < 0.001 in bar plots ( A – C , E – G )), and differences between individual groups were evaluated with the appropriate post hoc test. The results obtained for large-cell carcinoma were statistically insignificant ( D , H ). The images in ( I – Q ) show representative punches demonstrating the IHC reaction for POSTN in adenocarcinoma ( I – K ), squamous-cell carcinoma ( L – N ), and large-cell carcinoma ( O – Q ) with respect to the tumor grade. Error bars represent the standard deviation (SD). *** p < 0.001.

Techniques: Expressing, Standard Deviation

Mann–Whitney U test results for the differences between males and females. Only POSTN in stroma yielded statistically significant results.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Mann–Whitney U test results for the differences between males and females. Only POSTN in stroma yielded statistically significant results.

Techniques: Mann-Whitney U-Test

Correlation plots comparing POSTN expression in cells to POSTN expression in stroma, across all cancer types ( A ), in adenocarcinoma ( B ), in squamous-cell carcinoma ( C ), and in large-cell carcinoma ( D ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing POSTN expression in cells to POSTN expression in stroma, across all cancer types ( A ), in adenocarcinoma ( B ), in squamous-cell carcinoma ( C ), and in large-cell carcinoma ( D ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot.

Techniques: Expressing

Correlation plots comparing the expression of POSTN in stroma with the expression of pro-angiogenic markers, as quantified via the Weidner ( A – C ) or Chalkley ( D – F ) method. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in stroma with the expression of pro-angiogenic markers, as quantified via the Weidner ( A – C ) or Chalkley ( D – F ) method. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot.

Techniques: Expressing

Correlation plots comparing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) with the expression of pro-angiogenic markers, as quantified via the Chalkley method, assessed in adenocarcinoma. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot. The images in ( G – I ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) reaction for each individual protein, as previously described. The images in ( J – L ) show representative magnified regions of the microarray punches.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) with the expression of pro-angiogenic markers, as quantified via the Chalkley method, assessed in adenocarcinoma. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot. The images in ( G – I ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) reaction for each individual protein, as previously described. The images in ( J – L ) show representative magnified regions of the microarray punches.

Techniques: Expressing, Immunohistochemical staining, Microarray

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in cells ( A – C ) and in stroma ( D – F ) with the expression of pro-angiogenic markers, as quantified via the Chalkley method, assessed in squamous-cell carcinoma. The pro-angiogenic factors are CD31 ( A , D ), CD34 ( B , E ), and CD105 ( C , F ). The plots include p -values and R-values (calculated with Spearman’s correlation test) and the number of cases, which can be seen in the lower right corner of each plot. The images in ( G – I ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) staining for each individual protein, as previously described. The images in ( J – L ) show representative magnified regions of the microarray punches.

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Microarray

Correlation plots comparing the expression of POSTN in stroma ( A – D ) with the expression of VEGF-A, as assessed with the semi-quantitative IRS method, across all cancer types ( A ), in adenocarcinoma ( B ), in squamous-cell carcinoma ( C ), and in large-cell carcinoma ( D ). The images in ( E – G ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) staining for each individual protein, as previously described. The images in ( H – J ) show representative magnified regions of the microarray punches.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Correlation plots comparing the expression of POSTN in stroma ( A – D ) with the expression of VEGF-A, as assessed with the semi-quantitative IRS method, across all cancer types ( A ), in adenocarcinoma ( B ), in squamous-cell carcinoma ( C ), and in large-cell carcinoma ( D ). The images in ( E – G ) show punches from tissue microarrays demonstrating an example of the immunohistochemical (IHC) staining for each individual protein, as previously described. The images in ( H – J ) show representative magnified regions of the microarray punches.

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Microarray

Kaplan–Meier plots of survival for patients expressing POSTN in their cells ( A ), POSTN in their stroma ( B ), and VEGF-A ( C ) at levels below or above the median. The graphs in ( D , E ) show the plots of survival for patients with regard to the quartile classifications of the levels of POSTN expression in their cells ( D ) or stroma ( E ). Quartiles are shown in ascending order, with I representing the first 25% of results, II representing 26–50%, III representing 51–75%, and IV representing 76% and above. The significance of the differences was determined with the log-rank test. The p -value and the number of cases are given in the lower right corner of each plot.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Kaplan–Meier plots of survival for patients expressing POSTN in their cells ( A ), POSTN in their stroma ( B ), and VEGF-A ( C ) at levels below or above the median. The graphs in ( D , E ) show the plots of survival for patients with regard to the quartile classifications of the levels of POSTN expression in their cells ( D ) or stroma ( E ). Quartiles are shown in ascending order, with I representing the first 25% of results, II representing 26–50%, III representing 51–75%, and IV representing 76% and above. The significance of the differences was determined with the log-rank test. The p -value and the number of cases are given in the lower right corner of each plot.

Techniques: Expressing

Kaplan–Meier plots of survival for patients with POSTN in their cells ( A , B ) or stroma ( C , D ) with regard to the tumor type (adenocarcinoma ( A , C ) or squamous-cell carcinoma ( B , D )). The significance of the differences was determined using the log-rank test. The p -value and the number of cases are given in the lower right corner of each plot.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Kaplan–Meier plots of survival for patients with POSTN in their cells ( A , B ) or stroma ( C , D ) with regard to the tumor type (adenocarcinoma ( A , C ) or squamous-cell carcinoma ( B , D )). The significance of the differences was determined using the log-rank test. The p -value and the number of cases are given in the lower right corner of each plot.

Techniques:

Linear regression model constructed for the purpose of predicting survival days. It was determined that other predictors were not valid. The model yielded an R-value of 0.473 when the variables presented below were used.

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Linear regression model constructed for the purpose of predicting survival days. It was determined that other predictors were not valid. The model yielded an R-value of 0.473 when the variables presented below were used.

Techniques: Construct

Logistic regression model constructed for the prediction of 5-year survival. It was determined that other predictors were not significant. Measures of model effectiveness are shown on the ROC curve ( <xref ref-type=

Figure 13 )." width="100%" height="100%">

Journal: Cells

Article Title: Correlation between Periostin Expression and Pro-Angiogenic Factors in Non-Small-Cell Lung Carcinoma

doi: 10.3390/cells13171406

Figure Lengend Snippet: Logistic regression model constructed for the prediction of 5-year survival. It was determined that other predictors were not significant. Measures of model effectiveness are shown on the ROC curve ( Figure 13 ).

Techniques: Construct

Periostin and HIF-1α expression in thyroid cancer. (A) Periostin protein was localized in the cytoplasm of the cancer cells and in some normal thyroid cells, but with strong staining in the epithelial cancer cells. Brown color indicates periostin positivity, Scale bars, 25 µm. (B) PTC tissues exhibited a significantly higher expression of periostin as compared to normal thyroid tissues (P<0.01). Brown color indicates periostin positivity. (C) HIF-1α was localized in the cytoplasm of cancer cells and some normal thyroid cells, but with strong staining in the cancer cells. Brown color indicates HIF-1α positivity. Scale bars, 20 µm. (D) PTC tissues present with significantly higher expression of HIF-1α, as compared to normal thyroid tissues (P<0.01). (E) Images depicting the staining for HIF-1α and periostin were obtained from adjacent sections of the same tissue at varying magnifications. Scale bars, 25 µm. HIF-1α, hypoxia inducible factor 1 subunit α; PTC, papillary thyroid cancer.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Periostin and HIF-1α expression in thyroid cancer. (A) Periostin protein was localized in the cytoplasm of the cancer cells and in some normal thyroid cells, but with strong staining in the epithelial cancer cells. Brown color indicates periostin positivity, Scale bars, 25 µm. (B) PTC tissues exhibited a significantly higher expression of periostin as compared to normal thyroid tissues (P<0.01). Brown color indicates periostin positivity. (C) HIF-1α was localized in the cytoplasm of cancer cells and some normal thyroid cells, but with strong staining in the cancer cells. Brown color indicates HIF-1α positivity. Scale bars, 20 µm. (D) PTC tissues present with significantly higher expression of HIF-1α, as compared to normal thyroid tissues (P<0.01). (E) Images depicting the staining for HIF-1α and periostin were obtained from adjacent sections of the same tissue at varying magnifications. Scale bars, 25 µm. HIF-1α, hypoxia inducible factor 1 subunit α; PTC, papillary thyroid cancer.

Article Snippet: The primary antibodies used were as follows: Anti-HIF1α rabbit monoclonal antibody (cat. no. ab51608; Abcam; 1:500); anti-E-cadherin rabbit monoclonal antibody (cat. no. ab76319; Abcam; 1:1,000), anti-N-cadherin mouse monoclonal antibody (cat. no. ab98952; Abcam; 1:1,000); anti-periostin rabbit polyclonal antibody (cat. no. ab79946; Abcam; 1:500); anti-solute carrier family 5 member 5 (NIS) rabbit polyclonal antibody (cat. no. ab240588; Abcam; 1:1,500); anti-GAPDH mouse monoclonal antibody (cat. no. ab8245; Abcam; 1:1,000).

Techniques: Expressing, Staining

Hypoxia upregulates the expression of periostin in TC cells via HIF-1α. (A) Results of RT-qPCR analysis: the expression of HIF-1α mRNA in BCPAP cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (*P<0.05). Compared to transfection with periostin shRNA under normoxic conditions, the mRNA expression of HIF-1α in BCPAP cells significantly increased with periostin shRNA transfection under hypoxic conditions ( # P<0.05). n=3 biological replicates. Error bars represent standard deviation. (B) Results of RT-qPCR analysis: The mRNA expression of periostin in BCPAP cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (**P<0.01). The mRNA expression of periostin significantly decreased following transfection with periostin shRNA under normoxic conditions (*P<0.05). As compared to transfection with periostin shRNA under normoxic conditions, the mRNA expression of periostin in the BCPAP cells significantly increased following periostin shRNA transfection under hypoxic conditions ( # P<0.01). n=3 biological replicates. Error bars represent standard deviation. (C) Results of RT-qPCR analysis: The expression of HIF-1α mRNA in BCPAPs under hypoxic conditions for 24 h is significantly greater as compared normoxia (**P<0.01). The expression of HIF-1α mRNA significantly decreased following transfection with HIF-1α shRNA under normoxic conditions (*P<0.01). Compared to transfection with HIF-1α shRNA under normoxic conditions, the mRNA expression of HIF-1α in BCPAP cells increased following transfection with HIF-1α shRNA under hypoxic conditions ( # P<0.01). n=3 biological replicates. Error bars represent standard deviation. (D) Results of RT-qPCR analysis: The mRNA expression of periostin in BCPAP cells under hypoxic conditions for 24 h was significantly greater than under normoxic conditions (**P<0.01). The expression of periostin mRNA significantly decreased following the transfection of HIF-1α shRNA under normoxic conditions (*P<0.05). As compared to transfection with HIF-1α shRNA under normoxic conditions, the mRNA expression of periostin in the BCPAP cells significantly increased following transfection with HIF-1α shRNA under hypoxic conditions ( # P<0.01). n=3 biological replicates. Error bars represent standard deviation. (E) Western blot analysis of HIF-1α, N-cadherin, E-cadherin, periostin and NIS under normoxic or hypoxic conditions, with or without periostin shRNA transfection. (F) Western blot analysis of HIF-1α, N-cadherin, E-cadherin, periostin and NIS under normoxic or hypoxic conditions, with or without HIF-1α shRNA transfection. TC, thyroid cancer; HIF-1α, hypoxia inducible factor 1 subunit α; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NIS, solute carrier family 5 member 5.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Hypoxia upregulates the expression of periostin in TC cells via HIF-1α. (A) Results of RT-qPCR analysis: the expression of HIF-1α mRNA in BCPAP cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (*P<0.05). Compared to transfection with periostin shRNA under normoxic conditions, the mRNA expression of HIF-1α in BCPAP cells significantly increased with periostin shRNA transfection under hypoxic conditions ( # P<0.05). n=3 biological replicates. Error bars represent standard deviation. (B) Results of RT-qPCR analysis: The mRNA expression of periostin in BCPAP cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (**P<0.01). The mRNA expression of periostin significantly decreased following transfection with periostin shRNA under normoxic conditions (*P<0.05). As compared to transfection with periostin shRNA under normoxic conditions, the mRNA expression of periostin in the BCPAP cells significantly increased following periostin shRNA transfection under hypoxic conditions ( # P<0.01). n=3 biological replicates. Error bars represent standard deviation. (C) Results of RT-qPCR analysis: The expression of HIF-1α mRNA in BCPAPs under hypoxic conditions for 24 h is significantly greater as compared normoxia (**P<0.01). The expression of HIF-1α mRNA significantly decreased following transfection with HIF-1α shRNA under normoxic conditions (*P<0.01). Compared to transfection with HIF-1α shRNA under normoxic conditions, the mRNA expression of HIF-1α in BCPAP cells increased following transfection with HIF-1α shRNA under hypoxic conditions ( # P<0.01). n=3 biological replicates. Error bars represent standard deviation. (D) Results of RT-qPCR analysis: The mRNA expression of periostin in BCPAP cells under hypoxic conditions for 24 h was significantly greater than under normoxic conditions (**P<0.01). The expression of periostin mRNA significantly decreased following the transfection of HIF-1α shRNA under normoxic conditions (*P<0.05). As compared to transfection with HIF-1α shRNA under normoxic conditions, the mRNA expression of periostin in the BCPAP cells significantly increased following transfection with HIF-1α shRNA under hypoxic conditions ( # P<0.01). n=3 biological replicates. Error bars represent standard deviation. (E) Western blot analysis of HIF-1α, N-cadherin, E-cadherin, periostin and NIS under normoxic or hypoxic conditions, with or without periostin shRNA transfection. (F) Western blot analysis of HIF-1α, N-cadherin, E-cadherin, periostin and NIS under normoxic or hypoxic conditions, with or without HIF-1α shRNA transfection. TC, thyroid cancer; HIF-1α, hypoxia inducible factor 1 subunit α; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NIS, solute carrier family 5 member 5.

Techniques: Expressing, Quantitative RT-PCR, Transfection, shRNA, Standard Deviation, Western Blot, Real-time Polymerase Chain Reaction

Effects of hypoxia and periostin on the proliferation of TC cells. (A and B) Effects of hypoxia and periostin shRNA on proliferation were evaluated using CCK-8 assays in BCPAP and TPC-1 cells at different time points (24, 48 and 72 h). Results of CCK-8 assay at 72 h show significantly an increased cell proliferation of TC cells (BCPAP and TPC-1) under hypoxic conditions vs. normoxic conditions (P<0.05); transfection with Periostin shRNA inhibited the proliferation of cells (P<0.05). Hypoxia combined with transfection with periostin shRNA inhibited the proliferation of TC cells compared to the cells subjected only to hypoxia (P<0.05). n=3 biological replicates; error bars represent standard deviation. (C-F) Results of MTT assay: Both the BCPAP and TPC-1 cells exhibited a significantly greater proliferation under hypoxic compared with normoxic conditions for 3 and 5 days (*P<0.05); transfection with periostin shRNA inhibited cell proliferation following culture for 3 and 5 days (**P<0.01). Hypoxia combined with periostin shRNA transfection inhibited the proliferation of TC cells compared with the cells subjected only to hypoxia ( # P<0.01). n=3 biological replicates; error bars represent standard deviation. TC, thyroid cancer; CCK-8, Cell Counting Kit-8 assay.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Effects of hypoxia and periostin on the proliferation of TC cells. (A and B) Effects of hypoxia and periostin shRNA on proliferation were evaluated using CCK-8 assays in BCPAP and TPC-1 cells at different time points (24, 48 and 72 h). Results of CCK-8 assay at 72 h show significantly an increased cell proliferation of TC cells (BCPAP and TPC-1) under hypoxic conditions vs. normoxic conditions (P<0.05); transfection with Periostin shRNA inhibited the proliferation of cells (P<0.05). Hypoxia combined with transfection with periostin shRNA inhibited the proliferation of TC cells compared to the cells subjected only to hypoxia (P<0.05). n=3 biological replicates; error bars represent standard deviation. (C-F) Results of MTT assay: Both the BCPAP and TPC-1 cells exhibited a significantly greater proliferation under hypoxic compared with normoxic conditions for 3 and 5 days (*P<0.05); transfection with periostin shRNA inhibited cell proliferation following culture for 3 and 5 days (**P<0.01). Hypoxia combined with periostin shRNA transfection inhibited the proliferation of TC cells compared with the cells subjected only to hypoxia ( # P<0.01). n=3 biological replicates; error bars represent standard deviation. TC, thyroid cancer; CCK-8, Cell Counting Kit-8 assay.

Techniques: shRNA, CCK-8 Assay, Transfection, Standard Deviation, MTT Assay, Cell Counting

Effects of hypoxia and periostin on the invasiveness and migratory ability of TC cells. (A and B) Transwell migration and invasion assays. BCPAP and TPC-1 cells under normoxic or hypoxic conditions plus transfection with or without periostin shRNA (c-shRNA). The number of cells that migrated or invaded through Matrigel-coated inserts was determined. Invasiveness capability of cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA inhibited the invasiveness capability of cells (**P<0.01). Hypoxia combined with periostin shRNA transfection restored the invasiveness capability of cells compared to only periostin shRNA transfection ( ## P<0.01). The migratory capability of cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA inhibited the migration capability of cells (**P<0.01). Hypoxia combined with periostin shRNA transfection restored the migration capability of cells compared to only periostin shRNA transfection ( ## P<0.01). Scale bars, 50 µm; n=3 biological replicates. (C and D) Wound healing assay. BCPAP and TPC-1 cells under normoxia or hypoxia plus transfection with or without periostin shRNA. Wound healing assays suggested BCPAP cells under hypoxic conditions achieved an increased healing rate as compared with that under normoxic conditions (*P<0.05); periostin knockdown significantly inhibited this effect (**P<0.05). Hypoxia combined with periostin shRNA remitted the mobility of cells ( # P<0.05). Scale bars, 100 µm; n=3 biological replicates. TC, thyroid cancer.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Effects of hypoxia and periostin on the invasiveness and migratory ability of TC cells. (A and B) Transwell migration and invasion assays. BCPAP and TPC-1 cells under normoxic or hypoxic conditions plus transfection with or without periostin shRNA (c-shRNA). The number of cells that migrated or invaded through Matrigel-coated inserts was determined. Invasiveness capability of cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA inhibited the invasiveness capability of cells (**P<0.01). Hypoxia combined with periostin shRNA transfection restored the invasiveness capability of cells compared to only periostin shRNA transfection ( ## P<0.01). The migratory capability of cells under hypoxic conditions for 24 h was significantly greater than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA inhibited the migration capability of cells (**P<0.01). Hypoxia combined with periostin shRNA transfection restored the migration capability of cells compared to only periostin shRNA transfection ( ## P<0.01). Scale bars, 50 µm; n=3 biological replicates. (C and D) Wound healing assay. BCPAP and TPC-1 cells under normoxia or hypoxia plus transfection with or without periostin shRNA. Wound healing assays suggested BCPAP cells under hypoxic conditions achieved an increased healing rate as compared with that under normoxic conditions (*P<0.05); periostin knockdown significantly inhibited this effect (**P<0.05). Hypoxia combined with periostin shRNA remitted the mobility of cells ( # P<0.05). Scale bars, 100 µm; n=3 biological replicates. TC, thyroid cancer.

Techniques: Migration, Transfection, shRNA, Wound Healing Assay

Molecular mechanisms of the regulation of periostin expression by HIF-1α. (A) The human periostin promoter contains four HIF-1α consensus sequences for potential binding sites. (B) DNA agarose gel electrophoresis results of input, control IgG and amplified products by HIF-1α overexpression plasmid from primer 1 to primer 4. (C) The results of reverse transcription-quantitative PCR are presented as regions amplified with four different primer pairs (primer 1-primer 4). The results revealed that binding of HIF-1α in the region probed with primer 2 and primer 3 amplified significantly compared to the control group after HIF-1α overexpression (***P<0.001), and fold enrichment of primer 2 was higher. (D) Luciferase reporter assays results. Compared to the blank control group, periostin-promoter-WT co-transfection with HIF-1α overexpression plasmid significantly increased the luciferase activity (***P<0.001). Co-transfection of mutant periostin-promoter with primer 2 (Δ2 mutant-type) or primer 3 (Δ3 mutant-type) with a HIF-1α overexpression plasmid demonstrated a significantly decreased luciferase activity, as compared with periostin-promoter-WT ( # P<0.05 and ### P<0.001). Co-mutant of primer 2 and primer 3 (Δ2+3 mutant-type) resulted the luciferase activity being most significantly decreased ### P<0.001). HIF-1α, hypoxia inducible factor 1 subunit α; WT, wild-type.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Molecular mechanisms of the regulation of periostin expression by HIF-1α. (A) The human periostin promoter contains four HIF-1α consensus sequences for potential binding sites. (B) DNA agarose gel electrophoresis results of input, control IgG and amplified products by HIF-1α overexpression plasmid from primer 1 to primer 4. (C) The results of reverse transcription-quantitative PCR are presented as regions amplified with four different primer pairs (primer 1-primer 4). The results revealed that binding of HIF-1α in the region probed with primer 2 and primer 3 amplified significantly compared to the control group after HIF-1α overexpression (***P<0.001), and fold enrichment of primer 2 was higher. (D) Luciferase reporter assays results. Compared to the blank control group, periostin-promoter-WT co-transfection with HIF-1α overexpression plasmid significantly increased the luciferase activity (***P<0.001). Co-transfection of mutant periostin-promoter with primer 2 (Δ2 mutant-type) or primer 3 (Δ3 mutant-type) with a HIF-1α overexpression plasmid demonstrated a significantly decreased luciferase activity, as compared with periostin-promoter-WT ( # P<0.05 and ### P<0.001). Co-mutant of primer 2 and primer 3 (Δ2+3 mutant-type) resulted the luciferase activity being most significantly decreased ### P<0.001). HIF-1α, hypoxia inducible factor 1 subunit α; WT, wild-type.

Techniques: Expressing, Binding Assay, Agarose Gel Electrophoresis, Amplification, Over Expression, Plasmid Preparation, Real-time Polymerase Chain Reaction, Luciferase, Cotransfection, Activity Assay, Mutagenesis

Hypoxia induced the Warburg effect by upregulating the expression of periostin. (A) Results of phosphomolybdic acid colorimetry. ATP expression in BCPAP cells under hypoxic conditions was significantly lower than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA under normoxic conditions increased the ATP expression in BCPAPs (**P<0.01). As compared to the hypoxic group, transfection using periostin shRNA under hypoxic conditions significantly increased the ATP expression in BCPAP cells ( ## P<0.01). (B) Results of the LDH test. The LDH expression in BCPAPs under hypoxic conditions was significantly higher than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA under normoxic conditions decreased LDH expression in BCPAPs (**P<0.01). In comparison with the hypoxic group, transfection with periostin shRNA under hypoxic conditions significantly decreased LDH expression in BCPAP cells ( ## P<0.01). (C and D) The ECAR and OCR of BCPAP cells under different conditions were measured using the Seahorse analyzer. The ECAR of BCPAP cells under hypoxic conditions or with HIF-1α overexpression was significantly higher than that under nomoxia (***P<0.001). Transfection with periostin shRNA under normoxic conditions significantly decreased the ECAR of BCPAP cells (**P<0.01). Transfection using periostin shRNA under hypoxic conditions or co-transfection with HIF-1α overexpression plasmids increased the ECAR of BCPAP cells ( ### P<0.001). By contrast, the OCR of BCPAPs with HIF-1α overexpression was significantly decreased (**P<0.01). Transfection with periostin shRNA significantly elevated the OCR of BCPAP cells (*P<0.05). Transfection with periostin shRNA under hypoxic conditions or co-transfection with HIF-1α overexpression plasmids decreased the OCR of BCPAP cells ( ## P<0.01 and ### P<0.001, respectively). LDH, lactate dehydrogenase; ECAR, extracellular acidification rate; OCR, oxygen consumption rate; HIF-1α, hypoxia inducible factor 1 subunit α.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Hypoxia induced the Warburg effect by upregulating the expression of periostin. (A) Results of phosphomolybdic acid colorimetry. ATP expression in BCPAP cells under hypoxic conditions was significantly lower than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA under normoxic conditions increased the ATP expression in BCPAPs (**P<0.01). As compared to the hypoxic group, transfection using periostin shRNA under hypoxic conditions significantly increased the ATP expression in BCPAP cells ( ## P<0.01). (B) Results of the LDH test. The LDH expression in BCPAPs under hypoxic conditions was significantly higher than that under normoxic conditions (*P<0.05). Transfection with periostin shRNA under normoxic conditions decreased LDH expression in BCPAPs (**P<0.01). In comparison with the hypoxic group, transfection with periostin shRNA under hypoxic conditions significantly decreased LDH expression in BCPAP cells ( ## P<0.01). (C and D) The ECAR and OCR of BCPAP cells under different conditions were measured using the Seahorse analyzer. The ECAR of BCPAP cells under hypoxic conditions or with HIF-1α overexpression was significantly higher than that under nomoxia (***P<0.001). Transfection with periostin shRNA under normoxic conditions significantly decreased the ECAR of BCPAP cells (**P<0.01). Transfection using periostin shRNA under hypoxic conditions or co-transfection with HIF-1α overexpression plasmids increased the ECAR of BCPAP cells ( ### P<0.001). By contrast, the OCR of BCPAPs with HIF-1α overexpression was significantly decreased (**P<0.01). Transfection with periostin shRNA significantly elevated the OCR of BCPAP cells (*P<0.05). Transfection with periostin shRNA under hypoxic conditions or co-transfection with HIF-1α overexpression plasmids decreased the OCR of BCPAP cells ( ## P<0.01 and ### P<0.001, respectively). LDH, lactate dehydrogenase; ECAR, extracellular acidification rate; OCR, oxygen consumption rate; HIF-1α, hypoxia inducible factor 1 subunit α.

Techniques: Expressing, Colorimetric Assay, Transfection, shRNA, Comparison, Over Expression, Cotransfection

Schematic representation of the effect of hypoxia-HIF-1α-periostin axis in thyroid cancer. HIF-1α, hypoxia inducible factor 1 subunit α.

Journal: Oncology Reports

Article Title: Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer

doi: 10.3892/or.2024.8716

Figure Lengend Snippet: Schematic representation of the effect of hypoxia-HIF-1α-periostin axis in thyroid cancer. HIF-1α, hypoxia inducible factor 1 subunit α.

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